请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话:Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢!

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请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话:Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢!
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请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话:Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢!
请帮我解释一下overlapping subclone的意思,谢谢!
那请帮我翻译一句话:Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢!

请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话:Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢!
从意思上讲是“重叠亚克隆”,是指一组克隆/质粒中,每一个克隆的插入基因序列和另一个的序列间有重叠部分.
可以用在测序上,当一组插入序列都被测序之后,可以通过计算机参考重叠序列将所有结果拼接成一条大的完整序列.
依靠嵌套缺失法获得的重叠亚克隆,p1Dy10.1的开放读码框已被完整测序

重叠亚克隆

是亚克隆重叠(交错)的意思,应该是克隆形式的一种。